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KMID : 0545120030130060942
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Journal of Microbiology and Biotechnology
2003 Volume.13 No. 6 p.942 ~ p.948
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Phylogenetic Analysis of Phenanthrene-Degrading Sphingomonas
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HAN, KYUDONG
JUNG, YONG-TAE/SON, SEUNG-YEOL
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Abstract
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Soil samples were obtained from 5 sites contaminated with polycyclic aromatic hydrocarbons (PAHs). These soil samples were cultured in using phenanthrene as a sole carbon and energy source, and 36 strains of phenanthrene-degrading bacteria were isolated from 3 sites. Most of them degraded 500 ppm of phenanthrene within 8 to 10 days. and these isolates could degrade a few other PAHs other than phenanthrene. Their genotypes were determined by restriction digests of the 16s rRNA genes [amplified ribosomal DNA restriction analysis (ARDRA)]. It was found that all the phenanthrene degrading isolates were included i n 4 ARDRA types. and they showed a strict site endemism. 16s rDNAs of I2 strains selected from different sites were sequenced. and they were all confirmed as Sphingomonas strains. Their 16S rDNA sequences were compared (or phylogenetic analysis; their sequence showed a similar result to ARDRA typing. thus indicating that these heterotrophic soil bacteria are not regionally mixed. In addition, it was found that the inicrobial diversity among sampling sites could be monitored by 16s rDNA PCR-RFLP pattern alone. which is simpler and easier to perform, without 16s rDNA sequence analysis.
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